<p>Collected branches and leaves of willow were sent to a sequencing company for high-throughput sequencing to obtain high-quality genomic data. The data was stored on mobile hard drives and the National Biological Information Center database. After analyzing the transcription data of rot disease stress, 8 Xinjiang wild apple MYB, 1 EIL, and 1 ERF transcription factor genes were obtained by designing primers and cloning. After verification by the in situ transient expression gene function identification system, 10 disease resistance genes were identified. The gene was constructed into the plant overexpression vector pBI121-GFP and introduced into Escherichia coli (TOP10 strain) and glycerol bacteria and stored at-80℃. </p>
| collect time | 2022/01/01 - 2022/12/31 |
|---|---|
| collect place | Yili area |
| data format | form |
| Coordinate system |
self-contained
Plant samples are collected in the wild, DNA extracted, high-throughput sequencing and splicing to obtain genomic sequences. Gene primers are designed, amplified by PCR, obtained by base sequencing, and constructed into a plant overexpression vector.
An ab initio determination at the whole genome chromosome level was carried out for Salix cheilophila, and its continuity, consistency and integrity were evaluated in three dimensions. The total length of Salix cheilophila genome assembly is 343.04 Mb, and the length of the initially assembled contig N50 is 12.7 Mb. After filling the holes with the Oxford Nanopore ultra-long fragment, all 19 chromosomes in the genome have reached the level of seamless assembly. The length of the entire genome sequence reaches 16.66 Mb. It is the first genome in Salicaceae to achieve seamless assembly so far. The small reads sequenced by the Illumina platform were compared to the genome. The reads comparison rate was 99.04%, and the genome coverage rate was 99.71%, indicating that the assembled genome has good consistency. The integrity of the wild apple genome was evaluated using two internationally recognized conservative gene datasets, BUSCO and CEGMA, and a total of 98.7% and 95.6% complete single copy genes were assembled, indicating that the genome assembly results were relatively complete. The quality value (Qv) of evaluating Salix chinensis genome using the Merqury-mash software module was 42.76, which means the corresponding accuracy exceeded 99.99%(Qv = 40). To sum up, the Salix chinensis genome has reached a high level in terms of continuity, consistency, integrity and accuracy of assembly. Through the above evaluation methods, the data indicators obtained show that the wild apple genome assembly has good continuity and consistency, as well as high accuracy and integrity, and the data quality is qualified. The sequence data of 10 genes is the complete CDS region (nucleotide and amino acid sequences) of the gene, with a data size of 36 kb.
| # | number | name | type |
| 1 | 2021xjkk0500 | 2021xjkk0500 | National Science and technology support program |
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