采集乌柳枝条及叶片，送测序公司高通量测序获得高质量基因组数据，数据保存在移动硬盘和国家生物信息中心数据库。经腐烂病胁迫转录组数据分析，设计引物克隆获得8个新疆野苹果MYB、1个EIL、1个ERF转录因子基因，经原位瞬时表达基因功能鉴定体系验证，鉴定10个抗病基因。基因构建至植物过表达载体pBI121-GFP中，导入大肠杆菌（TOP10菌株）甘油菌-80℃低温保存。
", "ds_source": "自主产生
", "ds_process_way": "野外采集植物样本，进行DNA提取，高通量测序拼接，获得基因组序列。设计基因引物，PCR扩增，碱基测序获得，构建至植物过表达载体。
", "ds_quality": "针对乌柳（Salix cheilophila）进行了全基因组染色体水平的从头测定，对其连续性，一致性和完整度进行三个维度的评估。乌柳Salix cheilophila基因组拼装总长度为343.04 Mb, 基因组初步拼装的contig N50长度为12.7 Mb，使用Oxford Nanopore超长片段进行补洞后基因组中19条染色体均达到无缝拼装水平，全基因组序列N50长度达到16.66 Mb，是迄今杨柳科植物中首个实现无缝拼装的基因组。将Illumina平台测序的小片段reads比对到基因组，reads比对率为99.04%，基因组覆盖率为99.71%，说明组装的基因组有很好的一致性。使用国际公认的BUSCO和CEGMA两套保守基因数据集对野苹果基因组的完整度进行评估，共组装出98.7%和95.6%的完整单拷贝基因，说明基因组组装结果较完整。使用Merqury-mash软件模块评估乌柳基因组的质量值（quality value, Qv）为42.76，即对应准确度超过99.99% （Qv = 40）。 综上分析，乌柳基因组在拼装的连续性，一致性，完整性和准确度等方面均达到较高的水准。通过以上评估方法，得出的数据指标均表明：野苹果基因组组装具有较好的连续性和一致性，以及较高的准确度和完整度，数据质量合格。\n10个基因序列数据为基因完整CDS区（核苷酸和氨基酸序列），数据量大小为36 kb。
", "ds_acq_start_time": "2022-01-01 00:00:00", "ds_acq_end_time": "2022-12-31 00:00:00", "ds_acq_place": "伊犁地区", "ds_acq_lon_east": null, "ds_acq_lat_south": null, "ds_acq_lon_west": null, "ds_acq_lat_north": null, "ds_acq_alt_low": null, "ds_acq_alt_high": null, "ds_share_type": "login-access", "ds_total_size": 0, "ds_files_count": 0, "ds_format": "表格", "ds_space_res": null, "ds_time_res": "", "ds_coordinate": "无", "ds_projection": "", "ds_thumbnail": "83d7e020-5e92-48c3-924c-4fc44a145b8a.jpg", "ds_thumb_from": 2, "ds_ref_way": "", "paper_ref_way": "", "ds_ref_instruction": "天山野果林关键物种遗传资源数据集（2022年）来源于第三次新疆综合科学考察专项 \"天山野果林生态系统与生物多样性调查(2021xjkk0500)\"", "ds_from_station": null, "organization_id": "a5877b42-96ea-4f13-af7e-246f355413d6", "doi_value": "", "subject_codes": [ "180.51" ], "quality_level": 1, "publish_time": "2024-12-09 16:46:23", "first_publish_time": "2024-12-09 16:46:23", "last_updated": "2025-12-08 17:22:21", "protected": false, "protected_to": "2025-10-31 00:00:00", "lang": "zh", "cstr": "33110.11.XIEG.xjsedata.2022.00009107", "license": null, "extra": null, "files_shape": [], "features": null, "data_level": 0, "i18n": { "en": { "title": "Data set of genetic resources of key species in wild fruit forest in Tianshan Mountains (2022)", "ds_abstract": "Collected branches and leaves of willow were sent to a sequencing company for high-throughput sequencing to obtain high-quality genomic data. The data was stored on mobile hard drives and the National Biological Information Center database. After analyzing the transcription data of rot disease stress, 8 Xinjiang wild apple MYB, 1 EIL, and 1 ERF transcription factor genes were obtained by designing primers and cloning. After verification by the in situ transient expression gene function identification system, 10 disease resistance genes were identified. The gene was constructed into the plant overexpression vector pBI121-GFP and introduced into Escherichia coli (TOP10 strain) and glycerol bacteria and stored at-80℃.
", "ds_source": "self-contained
", "ds_process_way": "Plant samples are collected in the wild, DNA extracted, high-throughput sequencing and splicing to obtain genomic sequences. Gene primers are designed, amplified by PCR, obtained by base sequencing, and constructed into a plant overexpression vector.
", "ds_quality": "An ab initio determination at the whole genome chromosome level was carried out for Salix cheilophila, and its continuity, consistency and integrity were evaluated in three dimensions. The total length of Salix cheilophila genome assembly is 343.04 Mb, and the length of the initially assembled contig N50 is 12.7 Mb. After filling the holes with the Oxford Nanopore ultra-long fragment, all 19 chromosomes in the genome have reached the level of seamless assembly. The length of the entire genome sequence reaches 16.66 Mb. It is the first genome in Salicaceae to achieve seamless assembly so far. The small reads sequenced by the Illumina platform were compared to the genome. The reads comparison rate was 99.04%, and the genome coverage rate was 99.71%, indicating that the assembled genome has good consistency. The integrity of the wild apple genome was evaluated using two internationally recognized conservative gene datasets, BUSCO and CEGMA, and a total of 98.7% and 95.6% complete single copy genes were assembled, indicating that the genome assembly results were relatively complete. The quality value (Qv) of evaluating Salix chinensis genome using the Merqury-mash software module was 42.76, which means the corresponding accuracy exceeded 99.99%(Qv = 40). To sum up, the Salix chinensis genome has reached a high level in terms of continuity, consistency, integrity and accuracy of assembly. Through the above evaluation methods, the data indicators obtained show that the wild apple genome assembly has good continuity and consistency, as well as high accuracy and integrity, and the data quality is qualified.\nThe sequence data of 10 genes is the complete CDS region (nucleotide and amino acid sequences) of the gene, with a data size of 36 kb.
", "ds_acq_place": "Yili area", "ds_ref_instruction": "The genetic resource data set of key species in the Tianshan wild fruit forest (2022) comes from the third Xinjiang comprehensive scientific expedition special project \"Tianshan wild fruit forest ecosystem and biodiversity survey (2021 xjkk0500)\"", "ds_format": "form" } }, "license_type": null, "doi_reg_from": "reg_local", "cstr_reg_from": "reg_local", "doi_not_reg_reason": null, "cstr_not_reg_reason": null, "is_paper_in_submitting": false, "ds_topic_tags": [ "野果林", "DNA", "高通量测序" ], "ds_subject_tags": [ "植物学" ], "ds_class_tags": [], "ds_locus_tags": [ "伊犁地区" ], "ds_time_tags": [ 2022 ], "ds_contributors": [ "高贝", "刘晓洁" ], "ds_meta_authors": [ "高贝" ], "ds_managers": [ "高贝" ], "category": "生物多样性" }